Journal: Heliyon

Article Title: Acteoside attenuates acute lung injury following administration of cobra venom factor to mice

doi: 10.1016/j.heliyon.2022.e11622

Figure Lengend Snippet: Acteoside reduces inflammatory mediators and adhesion molecules in serum and BALF of ALI mice. Before administration with CVF to induced ALI, mice were orally pretreated with different concentrations (100, 50, and 20 mg/kg/day) of acteoside or PDTC (100 mg/kg/day) or vehicle for 7 days. Serum and BALF were collected 1 h after CVF (35 μg/kg) i.v. injection for the ELISA detection. A–C: IL-6, TNF-α, and ICAM-1 levels in serum; D–F: IL-6, TNF-α, and ICAM-1 in BALF. Results were presented as mean ± SEM ( n = 8). # P < 0.05 and ## P < 0.01 were compared with the control group; ∗ P < 0.05 and ∗∗ P < 0.01 were compared with the CVF group. ALI, acute injury lung; CVF, cobra venom factor; PDTC, pyrrolidinedithiocarbamic acid; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; ICAM-1, intracellular adhesion molecule; SEM, standard error of the mean.

Article Snippet: Cytokines in BALF and serum were determined with mouse ELISA kits (Boster Biological Technology Co., Ltd., Wuhan, China) for IL-6 (Cat. No. EK0411), TNF-α (Cat. No. EK0527), and ICAM-1 (Cat. No. EK0371).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Control, Combined Bisulfite Restriction Analysis Assay

Journal: International Journal of Molecular Sciences

Article Title: Exploring the Relationship of Relative Telomere Length and the Epigenetic Clock in the LipidCardio Cohort

doi: 10.3390/ijms20123032

Figure Lengend Snippet: Scattered plot depicting the linear correlation of DNAm age with the chronological age ( n = 779, R = 0.69).

Article Snippet: In summary: 500 ng of genomic leukocyte DNA sample underwent bisulfite conversion (EZ−96 DNA Methylation-Lightning Kit, ZYMO RESEARCH, Irvine, CA, USA) as described in the manufacturers’ protocol.

Techniques: